|Organization or Institution||University of Florida|
Synthetic "nanoreactors" to mimic biology
C. Adrian Figg, John B. Garrison, Brent S. Sumerlin
University of Florida
Polymerization-induced self-assembly (PISA) has been shown to be a useful technique in creating a wide array of polymer nanoparticles. The technique relies on chain-extending a water-soluble polymer block with a monomer that is water soluble, but the corresponding polymer is hydrophobic. This increasing amphiphilicity of the block copolymer induces self-assembly during the polymerization. In this work, a water-soluble poly(N,N-dimethyl acrylamide) (PDMA) macro-chain transfer agent (macro-CTA) is chain-extended with a copolymer of N,N-dimethyl acrylamide and diacetone acrylamide (DAAM) to synthesize polymeric vesicles containing proteins within the lumen of the vesicles. To achieve this, a small fraction of the PDMA macro-CTA is conjugated to a protein via a photodegradable linker. Following polymerization, the photodegradable linker can then be cleaved, and the polymer vesicles containing protein can be purified from the residual protein in solution, resulting in polymeric nanoreactors. Therefore, this report details the synthesis and characterization of protein-containing polymeric vesicles using PISA.