|Name||Miss Natalie Harvey|
|Organization or Institution||Florida Gulf Coast University|
|Topic||Biochemistry / Chem Bio.|
The Effects of Green Chemistry Gold Nanoparticles on the Development and Mortality of Zebrafish (Danio rerio) Embryos
Natalie Harvey, Adam Essler, and Amy Walker
Department of Chemistry, Florida Gulf Coast University, Fort Myers FL 33965, USA
The study of zebrafish embryo development and mortality while placed in incubation with gold nanoparticle solution gives great insight as to if green chemistry synthesized nanoparticles will be acceptable for use in the future on humans as a medication transport for cancer fighting drugs. These trials tested the effects of approximately 2nm through 10nm gold nanoparticles. The nanoparticle solutions created are all natural as they are made from fruit extract from bananas, kiwis, apples, and peaches. Over the course of a 5-day growth period of zebrafish embryos into fully formed fish, the embryos are monitored to determine if the effects of nanoparticles within their system result in harm to the embryo development. Our results indicated that the 2nm gold nanoparticles resulted in a lower mortality rate than that of the trials using 8nm gold nanoparticles. Those wells that house the highest concentration of nanoparticle solution resulted in a high percentage embryonic death by day 5. Overall, these trials proved to be successful in determining which size nanoparticles would be best for a medical application and which concentrations allowed into the biological system are tolerable before they become deadly.