|Name||Mr. Raja Reddy BommaReddy|
|Organization or Institution||UNIVERSITY OF SOUTH FLORIDA|
|Topic||Biochemistry / Chem Bio.|
Effect of novel atypical Protein Kinase C inhibitor (DNDA) on cell proliferation and migration of lung cancer cells
Raja Reddy BommaReddy, Rekha Patel, Mildred Acevedo-Duncan
Department of Chemistry, University of South Florida
Lung cancer is the second most common cancer and it is the leading cause of cancer death in both men and women. Lung cancer estimates from American Cancer Society in the united states for 2018 are about 234,030 new cases of lung cancer (121,680 in men and 112,350 in women) and 154,050 deaths from lung cancer (83,550 in men and 70,500 in women). PKC isozymes play important roles in the development and progression of many cancers by regulating cell cycle, survival, apoptosis, cell motility and malignant transformation. The efforts to develop a selective drug to target a specific PKC is challenging due to the complexity of the second messenger systems that involve PKCs. Our focus is to study the role of atypical PKCs in the cell proliferation and migration in lung cancer cell lines. Our hypothesis is DNDA inhibits the cell proliferation and migration of lung cancer cells. We used a novel non-specific inhibitor of aPKCs namely DNDA (3,4-amino-2,7napthalenedisulfonic acid). Our preliminary data from cell viability studies using cellometer and WST-1 assay showed significant reduction in the cell proliferation with 10µM DNDA treatment. Western blot results showed that the phosphorylation of PKC-i and phosphorylation of FAK are decreased in A549 lung cancer cells upon treatment with DNDA. Immunoprecipitation (IP) data revealed there is an association of PKCι with FAK and cleaved FAK fragments are observed. Elevated levels of FAK is implicated in the progression of cancer and plays a vital role in the invasion and migration of the cancer. Wound healing assay showed that DNDA treatment reduced the migration of the A549 lung cancer cells when compared to the control. Based on our results, DNDA is good candidate to explore the pathways by which it is inhibiting the migration of lung cancer cells.